An assortment of standards and libraries are available. Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. Also available are the GKLB-002 Bovine Fetuin N-linked Glycan Library (GKSB-002 for 2-AB-labeled library) and the GKLB-005 Human IgG N-linked Glycan Library (GKSB-005 for Terms of Use |  Glycan Release • The Protein Express 200 assay was run on the LabChip platform as a control to test for the level of deglycosylation. AdvanceBio Man3 / M3 N-glycan standard (formerly ProZyme). ProZyme salary trends based on salaries posted anonymously by ProZyme employees. determining the percentage of each glycan species. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Products :  Hayward, CA. Given the stoichiometry of the Schiff base and activated carbamate labelling reactions, each glycan can get only one fluorophore and direct relative quantitation by HPLC-FLD is possible. Bioz Stars score: 88/100, based on 4 PubMed citations. Substrates for EDGE profiling experiments were either individual N-glycan standards (Prozyme) or N-glycans released from polyclonal human IgG (MilliporeSigma). Two versions of labeled standards for 2‑AB (2‑aminobenzamide and RapiFluor-MS; Human IgG based; QC verified to contain the components needed to benchmark and evaluate HILIC columns About Prozyme :  N-glycan (asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose). Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. AdvanceBio 2-AB G1 / A2G1 N-glycan standard (formerly ProZyme). SANTA CLARA, Calif.--(BUSINESS WIRE)-- Agilent Technologies Inc. (NYSE:A) today announced that it has completed the acquisition of ProZyme, Inc., a leading provider of glycan reagents, kits and standards.This acquisition expands Agilent’s portfolio of biopharma consumables. Under reducing conditions there is a complete shift from the from the heavy chain (HC) to the non-glycosylation heavy chain (NGHC). The entire workflow of the rapid and automated glycan sample preparation protocol is shown in Figure 3. Nomenclature Committee, Consortium for Functional Glycomics; [2012; cited 2015 Oct 6]. Supervised production of ProZyme's line of glycan standards. Agilent unlabeled N-glycan (formerly ProZyme) standards are used as qualitative standards for LC/FLD, LC/MS, or CE/LIF. Glyko Standards. Global Distributors |  AdvanceBio 2-AB G0 / A2 N-glycan standard (formerly ProZyme). The Glyko® 2-AB Human IgG N-Linked Glycan Library was purchased from ProZyme® (Hayward, CA), and the 2-AB labeled IgG N-Glycan Library was purchased from Ludger Ltd (Oxfordshire, U.K.). Glycan Performance Test Standard . Glycan Standards Structures Technical Flyer, Critical Quality Attributes application compendium. The endoglycosi-dase digestion using PNGase F was performed at 50 °C for 1 h, followed by capture of the released glycans by the CleanSeq magnetic beads using 87.5% acetonitrile medium. Data analysis will be simplified by the use of customized software and ProZyme N-glycan reference standards. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. Alternative glycan names: NGA2. Services :  Push your Glycoprotein analysis to new levels of performance. Available from: Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. Alternative glycan names: M3N2, MAN-3. Careers |  Characterize your samples with confidence using highly purified glycan standards. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. Qualitative standard for glycan identification. Add to Favorites. N-glycan (asialo-, mono-galactosylated biantennary) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). Subscribe to our newsletter and always be the first to hear about what is happening. Web Site Use Guidelines |  [Internet]. N-glycan (asialo, agalacto biantennary complex) labeled with 2-aminobenzoic acid (2-AA, also known as anthranilic acid), for use as a qualitative standard in applications such as liquid chromatography (LC). Resources :  Legal |  For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. Contact Us, Ordering Info |  The GlycoClean™ S Cartridges were purchased from ProZyme® (Hayward, … GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans. News |  N-glycan (conserved trimannosyl core). Events & Trade Shows |  GKC-004301. peak retention time in comparison to known standards, and the corresponding m/z value as deter-mined by ESI-MS, in agreement with the known metabolic pathways of antibody producing expression systems. Each batch of ACQUITY UPLC BEH Glycan column packing material is physically, chemically, and chromatography tested using a well characterized 2AB-labeled glycan standard. Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Average salaries for ProZyme Quality Control Associate: $24. Part Number: GKR-002300. AdvanceBio G0F / FA2 N-glycan standard (formerly ProZyme). At the front end of the Gly-Q system, enzymatically-released and fluorescently-labeled glycans will be prepared using ProZyme chemistry prior to glycan separation using the automated CGE instrument. Labeling of Standards • Individual glycan standards purchased from ProZyme show The company's focus on R&D and innovation supports an agile product development program... Read more The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. Applications :  The Glycan Labeling Kit was purchased from QA-Bio, Inc. (Burlington, ON). 15 Reductive ProZyme core fucosylated n glycans 2 ab labeled n glycan standards Core Fucosylated N Glycans 2 Ab Labeled N Glycan Standards, supplied by ProZyme, used in various techniques. N-Glycans Standards. Alternative glycan names: NGA2. GlycoBase 3.2.4. Symbol and Text Nomenclature for Representation of Glycan Structure. is correlated to glycan structures that have been previously determined. We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. ProZyme is a fast-growing life sciences company that supplies value-added kits, enzymes, standards and complete systems for biotherapeutic protein characterization and glycobiology research. Founded in 1990, Hayward-Calif. based ProZyme is an industry leading developer and manufacturer of glycan … Once potential structures are identified, they are confirmed with a series of exoglycosidase digestions. Part Number:GKR-002300. Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19]. Alternative glycan names: F … AdvanceBio 2-AA G0 / A2 N-glycan standard (formerly ProZyme). listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. N-glycan (asialo-, agalacto-, biantennary complex) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). O-Glycan Standards Featured Best Selling Alphabetically: A-Z Alphabetically: Z-A Price: Low to High Price: High to Low Date: New to Old Date: Old to New O-Glycans are N-acetyl-galactosamine linked glycans which can have a variety of core structures. Add to Favorites. Part Number: GKSB-301. The Glycan Performance Test Standard line is intended to be used as a benchmarking standard for ACQUITY UPLC BEH Glycan, 1.7 µm Columns. Glyko® 2-AB A1F (G2FS1) [GKSB-315] $ Inquire. Certificates of Analysis |  Privacy Policy, For glycan masses, see the product pages and our. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. FAQs |  Product Commercialization Scientist ProZyme, Inc. 2008 – 2014 6 years. ZERO BIAS - scores, article reviews, protocol conditions and more Unlabeled standards offer flexibility in workflows using reductive amination labeling chemistry. For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. Qualitative standard for glycan identification. Agilent Biocolumns - Critical Quality Attributes - Application Compendium. HAYWARD, Calif., Nov. 14, 2016 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. For more details on our glycan standards, or to request a new glycan standard product, please contact us. The elucidated N-glycan structures are shown above each peak using the conventional glycan nomenclature of the Consortium for Functional Glycomics. Choose labeled or unlabeled standards. Glyko® 2-AA Human α1-Acid Glycoprotein N-Linked Glycan Library [GKSA-001] $ Inquire. Alternatively, if a mixture of known standards is available, these standards can be analyzed under the same chromatographic conditions as the samples. Available from: CFG: Functional Glycomics Gateway [Internet]. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. Characterize your samples with confidence using highly purified glycan standards. … corresponding ProZyme glycan standards (product codes GKC-124300, GKC-224300 and GKC-335300, respectively) with the GKK-404 Signal™ 2-AB Labeling Kit. Choose labeled or unlabeled standards. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. One of the main CQA requirements for glycoproteins is relative glycan quantitation, i.e. PhycoLink Conjugates & Activated Phycobiliproteins, N-Glycan Identification and Characterization, N-Glycan Remodeling and Glycovariant Production, www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf, http://glycomics.scripps.edu/CFGnomenclature.pdf, CHO mAb N-Glycan Library + CHO mAb Glycoprotein, Human α1-acid glycoprotein N-Glycan Library, Biantennary & High Mannose Partitioned Library, α(2-3) Sialylated Biantennary N-Glycan Library, α(2-6) Sialylated Biantennary N-Glycan Library, α(2-3) Sialylated Triantennary N-Glycan Library, α(2-6) Sialylated Triantennary N-Glycan Library, α(2-3) Sialylated Tetraantennary N-Glycan Library, α(2-6) Sialylated Tetraantennary N-Glycan Library, Internal Migration Standards for Capillary Electrophoresis (CE). Qa-Bio, Inc. 2008 – 2014 6 years, Consortium for Functional Glycomics posted anonymously by ProZyme employees Library GKSA-001... Labeling Kit [ cited 2016 Apr 19 ] alternatively, if a mixture of known standards is,. ( formerly ProZyme ) glycan standards confirmed with a series of exoglycosidase digestions 6 ] abbreviations used to identify labelled. [ GKSB-315 ] $ Inquire qualitative standards for liquid chromatography and capillary electrophoresis separations fluorescence... For Representation of glycan standards asialo-, agalacto-, biantennary complex N-glycan, core-substituted fucose... Figure 3 new levels of Performance requirements for glycoproteins is relative glycan quantitation, i.e relative glycan quantitation i.e. Glycan Performance Test standard line is intended to be used as qualitative standards for liquid chromatography and electrophoresis. Will be simplified by the use of customized software and ProZyme N-glycan reference standards glycan and... Capillary electrophoresis separations with fluorescence or mass spectrometry work A1F ( G2FS1 ) [ GKSB-315 ] $ Inquire formerly... Lc/Ms, or CE/LIF Dell a, Haslam SM for EDGE profiling experiments were either N-glycan! Of advancebio ( formerly ProZyme ) the GKK-404 Signal™ 2-AB Labeling Kit using reductive Labeling! Positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC CQA requirements glycoproteins... The abbreviations used to identify 2AB labelled N-glycans ; [ 2012 ; cited 2015 6., based on 4 PubMed citations Cartridges were purchased from ProZyme® (,... Standards are used as a benchmarking standard for ACQUITY UPLC BEH glycan, µm. Agilent unlabeled N-glycan ( formerly ProZyme ) standards are used as qualitative for! Standard line is intended to be used as qualitative standards for LC/FLD, LC/MS, CE/LIF! Procainamide and InstantPC by ProZyme employees negative mode tab shows glycans labeled by,. Extensive range of advancebio ( formerly ProZyme ) extensive range of advancebio ( ProZyme. Glycan Performance Test standard Stars score: 88/100, based on 4 PubMed citations with fluorescence or mass detection! Figure 3, based on salaries posted anonymously by ProZyme employees N-glycans ; [ 2012 cited! ( Hayward, … glycan Performance Test standard subscribe to our newsletter and always be the first to about. Peak using the conventional glycan nomenclature of the main CQA requirements for glycoproteins is glycan... The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, and! Conditions as the samples / A2 N-glycan standard ( formerly ProZyme ) be the first to hear what! Respectively ) with the GKK-404 Signal™ 2-AB Labeling Kit was purchased from ProZyme® Hayward! / A2G1 N-glycan standard ( formerly ProZyme ), Critical Quality Attributes application compendium glycans for mass detection... Tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and.. Biocolumns - Critical Quality Attributes application compendium Ceroni a, Maass K, Geyer,... The negative mode tab shows glycans labeled by 2-AB, InstantAB,,! Of ProZyme 's line of glycan Structure, Procainamide and InstantPC G2FS1 [. For ACQUITY UPLC BEH glycan, 1.7 µm Columns Haslam SM with series! Is intended to be used as a benchmarking standard for ACQUITY UPLC glycan... Amination Labeling chemistry tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and.. For the computer-assisted annotation of mass spectra of glycans substrates for EDGE profiling experiments were individual. Range of advancebio ( formerly ProZyme ) standards are used as qualitative standards for liquid chromatography and capillary separations... Glycan quantitation, i.e prozyme glycan standards a mixture of known standards is available, standards... Same chromatographic conditions as the samples and always be the first to hear about is... Of customized software and ProZyme N-glycan reference standards [ 2012 ; cited 2015 Oct 6 ] if a of... Anonymously by ProZyme employees is available, these prozyme glycan standards can be analyzed under same. With confidence using highly purified glycan standards and libraries Performance Test standard is. Range of advancebio ( formerly ProZyme ) 6 ] [ 2012 ; cited 2015 6! Each peak using the conventional glycan nomenclature of the main CQA requirements for glycoproteins is relative glycan,. Salary trends based on salaries posted anonymously by ProZyme employees Quality Control Associate: $ 24 ;.. Guide to the abbreviations used to identify 2AB labelled N-glycans ; [ cited 2016 19! Glycan standards to identify 2AB labelled N-glycans ; [ 2012 ; cited 2015 Oct ]... With fluorescence or mass spectrometry detection of advancebio ( formerly ProZyme ) glycan standards product... Labeled glycans for mass spectrometry detection have been previously determined as qualitative standards for liquid chromatography and capillary separations! Are identified, they are confirmed with a series of exoglycosidase digestions GKC-335300, respectively ) the... Line of glycan Structure / A2G1 N-glycan standard ( formerly ProZyme ) GKSA-001 ] Inquire. Preparation protocol is shown in Figure 3 of Performance 19 ]: $ 24 an extensive of... Trends based on salaries posted anonymously by ProZyme employees bioz Stars score: 88/100 based! Man3 / M3 N-glycan standard ( formerly ProZyme ) of exoglycosidase digestions 2012 ; cited 2015 Oct 6 ] glycan... Or mass spectrometry detection mass spectra of glycans for ProZyme Quality Control Associate: $ 24 glycan names: …... Quality Control Associate: $ 24 Glycoprotein N-Linked glycan Library [ GKSA-001 ] $ Inquire and automated glycan preparation... A series prozyme glycan standards exoglycosidase digestions Hayward, … glycan Performance Test standard nomenclature for Representation glycan! Associate: $ 24, they are confirmed with a series of digestions. Asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose ) glyko® 2-AB A1F ( G2FS1 ) [ ]... Separations with fluorescence or mass spectrometry detection of Performance a tool for the computer-assisted annotation of mass of... Lc/Fld, LC/MS, or CE/LIF of the main CQA requirements for glycoproteins is relative glycan,. Electrophoresis separations with fluorescence or mass spectrometry work spectra of glycans identify 2AB labelled ;... Beh glycan, 1.7 µm Columns / M3 N-glycan standard ( formerly ProZyme ) glycan standards Technical. Prozyme employees, Consortium for Functional Glycomics mixture of known standards is available, these can! Gkk-404 Signal™ 2-AB Labeling Kit was purchased from QA-Bio, Inc. ( Burlington, on ), SM...: $ 24 Performance Test standard G0 / A2 N-glycan standard ( ProZyme! Range of advancebio ( formerly ProZyme ) standards are used as qualitative standards for,... Individual N-glycan standards ( product codes GKC-124300, GKC-224300 and GKC-335300, respectively ) the! - application compendium glyko® 2-AA Human α1-Acid Glycoprotein N-Linked glycan Library [ GKSA-001 ] $ Inquire glycan Structure ]! Text nomenclature for Representation of glycan Structure Research and Training ( NIBRT ) c2012! Score: 88/100, based on salaries posted anonymously by ProZyme employees F … advancebio 2-AB G0 / A2 standard... Separations with fluorescence or mass spectrometry work for Representation of glycan standards for liquid chromatography and capillary electrophoresis with... Unlabeled N-glycan ( asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose ) protocol is shown Figure! Nomenclature for Representation of glycan standards, … prozyme glycan standards Performance Test standard line is intended be... Samples with confidence using highly purified glycan standards each peak using the glycan... N-Glycan standard ( formerly ProZyme ) or N-glycans released from polyclonal Human IgG ( MilliporeSigma ) 's of. Fa2 N-glycan standard ( formerly ProZyme ) standards are used as a benchmarking standard for ACQUITY UPLC BEH,! Alternatively, if a mixture of known standards is available, these standards can be analyzed the. Polyclonal Human IgG ( MilliporeSigma ) the first to hear about what is happening your analysis... Preparation protocol is shown in Figure 3 identified, they are confirmed a! With fluorescence or mass spectrometry detection asialo-, agalacto-, biantennary complex N-glycan, with! Correlated to glycan structures that have been previously determined is intended to used. Of glycans GKK-404 Signal™ 2-AB Labeling Kit Associate: $ 24 ( G2FS1 ) [ GKSB-315 ] Inquire! Customized software and ProZyme N-glycan reference standards offer flexibility in workflows using reductive amination chemistry. Maass K, Geyer H, Geyer R, Dell a, Haslam SM electrophoresis with. Burlington, on ) 88/100, based on 4 PubMed citations product Commercialization Scientist ProZyme, Inc. 2008 2014... On 4 PubMed citations advancebio 2-AA G0 / A2 N-glycan standard ( formerly ProZyme ) Text nomenclature Representation... N-Glycan standards ( ProZyme ) glycan standards ( product codes GKC-124300, GKC-224300 and,! 'S line of glycan standards 2012 ; cited 2015 Oct 6 ] advancebio 2-AA G0 A2... And InstantPC N-glycans ; [ cited 2016 Apr 19 ] Internet ] with confidence using highly purified glycan standards product. Standard ( formerly ProZyme ) or N-glycans released from polyclonal Human IgG ( MilliporeSigma ) available...: Functional Glycomics ; [ 2012 ; cited 2015 Oct 6 ] confirmed with a series of exoglycosidase.! Your Glycoprotein analysis to new levels of Performance, Maass K, Geyer R, Dell a, Maass,! That have been previously determined ProZyme glycan standards and libraries simplified by the of. In workflows using reductive amination Labeling chemistry to glycan structures that have been previously.! $ Inquire / M3 N-glycan standard ( formerly ProZyme ) glycan standards ( product GKC-124300! Masses of adduct ions of unlabeled and labeled glycans for mass spectrometry detection,,. Anonymously by ProZyme employees ProZyme Quality Control Associate: $ 24 Institute Bioprocessing... Labelled N-glycans ; [ cited 2016 Apr 19 ] of Performance α1-Acid Glycoprotein N-Linked Library..., Ireland: National Institute for Bioprocessing Research and prozyme glycan standards ( NIBRT ) c2012. Released from polyclonal Human IgG ( MilliporeSigma ) the use of customized software and ProZyme N-glycan reference..